英文:
Problems when running GDC_prepare in R
问题
我尝试从GDC检索和准备一些数据，我正在运行以下代码，我是从这个YouTube视频中复制粘贴的：video。
以下是代码：
library(TCGAbiolinks)library(tidyverse)library(maftools)library(pheatmap)library(SummarizedExperiment)# 获取项目列表gdcprojects <- getGDCprojects()# 构建查询query_TCGA <- GDCquery(project = 'TCGA-BRCA',data.category = 'Transcriptome Profiling')output_query_TCGA <- getResults(query_TCGA)# 构建查询以检索基因表达数据query_TCGA <- GDCquery(project = 'TCGA-BRCA',data.category = 'Transcriptome Profiling',experimental.strategy = 'RNA-Seq',workflow.type = 'STAR - Counts',access = 'open',barcode = c('TCGA-LL-A73Y-01A-11R-A33J-07', 'TCGA-E2-A1IU-01A-11R-A14D-07', 'TCGA-AO-A03U-01B-21R-A10J-07'))getResults(query_TCGA)# 下载数据 - GDCdownloadGDCdownload(query_TCGA)# 准备数据tcga_brca_data <- GDCprepare(query_TCGA)
在运行代码的最后一行之后，我收到以下错误消息：
leted after 0 sError in `map()`:i In index: 1.i With name: 1.Caused by error in `y[, 2:4]`:! Can't subset columns past the end.i Locations 2, 3, and 4 don't exist.i There is only 1 column.Run `rlang::last_error()` to see where the error occurred.Warning messages:1: One or more parsing issues, call `problems()` on your data frame for details, e.g.:dat <- vroom(...)problems(dat)2: One or more parsing issues, call `problems()` on your data frame for details, e.g.:dat <- vroom(...)problems(dat)3: One or more parsing issues, call `problems()` on your data frame for details, e.g.:dat <- vroom(...)problems(dat)
我不理解这个错误是什么原因，因为我正在使用与视频中相同的代码。
英文:
I'm trying to retrieve and prepare some data from GDC and I'm running the following code which I copy-pasted from this YouTube video:video.
Below is the code:
library(TCGAbiolinks)library(tidyverse)library(maftools)library(pheatmap)library(SummarizedExperiment)# get a list of projectsgdcprojects <- getGDCprojects()# building a queryquery_TCGA <- GDCquery(project = 'TCGA-BRCA',data.category = 'Transcriptome Profiling')output_query_TCGA <- getResults(query_TCGA)# build a query to retrieve gene expression data ------------query_TCGA <- GDCquery(project = 'TCGA-BRCA',data.category = 'Transcriptome Profiling',experimental.strategy = 'RNA-Seq',workflow.type = 'STAR - Counts',access = 'open',barcode = c('TCGA-LL-A73Y-01A-11R-A33J-07', 'TCGA-E2-A1IU-01A-11R-A14D-07','TCGA-AO-A03U-01B-21R-A10J-07'))getResults(query_TCGA)# download data - GDCdownloadGDCdownload(query_TCGA)# prepare datatcga_brca_data <- GDCprepare(query_TCGA)
And after running the last line of code I get the following error message:
leted after 0 sError in `map()`:i In index: 1.i With name: 1.Caused by error in `y[, 2:4]`:! Can't subset columns past the end.i Locations 2, 3, and 4 don't exist.i There is only 1 column.Run `rlang::last_error()` to see where the error occurred.Warning messages:1: One or more parsing issues, call `problems()` on your data frame for details, e.g.:dat <- vroom(...)problems(dat)2: One or more parsing issues, call `problems()` on your data frame for details, e.g.:dat <- vroom(...)problems(dat)3: One or more parsing issues, call `problems()` on your data frame for details, e.g.:dat <- vroom(...)problems(dat)
I do not understand what is causing this error since I'm using the same code as in the video.
答案1
得分: 0
跨贴并在https://support.bioconductor.org/p/9149780/上回答。
解决方案：更新软件包。
英文:
Cross-posted and answered at https://support.bioconductor.org/p/9149780/
Solution: Update packages.
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