英文:
Nextflow script with both 'local' and 'awsbatch' executor
问题
我有一个在AWS Batch中执行的Nextflow流水线。最近,我尝试添加一个从本地计算机上传文件到S3存储桶的流程,这样我就不必在每次运行之前手动上传文件了。我编写了一个处理上传的Python脚本,并将其包装成一个Nextflow流程。因为我是从本地计算机上传的,所以我希望上传流程使用executor 'local'
。
这需要启用Fusion文件系统,以便在S3中拥有工作目录。但是当我启用Fusion文件系统时,我无法访问本地文件系统。在我理解中,启用Fusion文件系统时,任务在Wave容器中运行,无法访问主机文件系统。有没有人有使用启用FusionFS的Nextflow并如何访问主机文件系统的经验?谢谢!
英文:
I have a Nextflow pipeline executed in AWS Batch. Recently, I tried to add a process that uploads files from local machine to S3 bucket so I don't have to upload files manually before each run. I wrote a python script that handles the upload and I wrapped it into a Nextflow process. Since I am uploading from a local machine, I want the upload process with
executor 'local'
This requires a Fusion filesystem enabled in order to have a Work Dir in S3. But when I enable the Fusion filesystem I don't have access to my local filesystem. In my understanding, when Fusion filesystem is enabled, the task runs in Wave container without access to host filesystem. Does anyone have experience with running Nextflow with FusionFS enabled and how to access host filesystem? Thanks!
答案1
得分: 1
I don't think you need to manage a 混合工作负载 here. Pipeline inputs can be stored either locally or in an S3 bucket. If your files are stored locally and you specify a working directory in S3, Nextflow will already try to upload them into the staging area for you. For example, if you specify your working directory in S3 using -work-dir 's3://mybucket/work'
, Nextflow will try to stage the input files under s3://mybucket/work/stage-<session-uuid>
. Once the files are in the staging area, Nextflow can then begin to submit jobs that require them.
Note that a Fusion file system is not strictly required to have your working directory in S3. Nextflow includes support for S3. Either include your AWS access and secret keys in your pipeline configuration or use an IAM role to allow your EC2 instances full access to S3 storage.
英文:
I don't think you need to manage a hybrid workload here. Pipeline inputs can be stored either locally or in an S3 bucket. If your files are stored locally and you specify a working directory in S3, Nextflow will already try to upload them into the staging area for you. For example, if you specify your working directory in S3 using -work-dir 's3://mybucket/work'
, Nextflow will try to stage the input files under s3://mybucket/work/stage-<session-uuid>
. Once the files are in the staging area, Nextflow can then begin to submit jobs that require them.
Note that a Fusion file system is not strictly required to have your working directory in S3. Nextflow includes support for S3. Either include your AWS access and secret keys in your pipeline configuration or use an IAM role to allow your EC2 instances full access to S3 storage.
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